The initiation of public health nursing in China in 1920s was a result of the transnational flow of people, knowledge and culture. Transnational educational institutions and non-governmental organizations, represented by Peking Union Medical College (PUMC) and the Rockefeller Foundation, as well as by individuals, played a dominant role in shaping the initiation and development of public health in China in the 1920s to 1930s. PUMC was the hub to disseminate its founder's vision and model in public health in China through integration of education with empirical initiatives in public health. Nursing education programs of the School of Nursing at PUMC provided expertise, human resources, and leadership in public health in China from the 1920s until the beginning of the 1950s. Throughout this time, as a profession predominated by women, public health nursing served as a good example to demonstrate women's role in the transnational flow of people, knowledge, and culture.